New manuscript posted to bioRxiv
Congratulations to João Silva and everyone else in the lab who contributed to the first manuscript arising from his masters work. The manuscript, “Plasmids for independently tunable, low-noise expression of two genes,” is now available on bioRxiv. We characterized new plasmids that can be combined to tune the expression of two different genes in E. coli, and we discuss how these plasmids can be used to improve a variety of microbiology experiments. We also showed some data for single-molecule mRNA detection using the bacteriophage PP7 coat protein. The figure below shows how we can use this system to change the expression levels of GFP and mScarlet-I independently.
The manuscript was written using Manubot, a revolutionary tool for composing scientific manuscripts online.
