New plasmids for low-noise gene expression at AddGene
New plasmids from the lab are now available at AddGene. First, a collection of plasmids for RNA imaging in E. coli using the coat protein from the bacteriophage PP7 fused to different fluorescent proteins. These are based on work from a few papers from Robert Singer’s lab, and make it possible to observe single RNAs containing tandem repeats of a specific sequence with either a cyan or red fluorescent protein. Second, plasmids from João Silva’s Masters project are available for low-noise gene expression. See more information on those in this Twitter thread:
Here's some new plasmids that use autoregulation and bicistronic expression to achieve tunable, low noise gene expression (rather than all-or-none expression that you get with many bacterial expression systems) - https://t.co/mX9i4yFDVM
— Zach Hensel (@alchemytoday) December 5, 2018
