New paper published in mSphere
May 29, 2019
João Silva’s Masters research culimated in a paper published in mSphere today: Plasmids for Independently Tunable, Low-Noise Expression of Two Genes. The paper reports the development and characterization of two compatible plasmids that we co-transformed into E. coli to independently induce GFP (GFPmut2) and RFP (mScarlet-I) expression. Together with Soraia Lopes and Diogo Grilo in the lab, we also showed how the new IPTG-inducible plasmid that João created can be used for imaging single mRNA molecules.
